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317 ve cadherin  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology 317 ve cadherin
    317 Ve Cadherin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 2. RBL2 regulates cell fate specification between neuroepithelium and neural crest (A) RBL2 is necessary for neuroectoderm formation. Immunostaining of neuroectoderm markers in Scramble and RBL2 KD cells. Scale bar, 100 mm. (B–D) RBL2 controls the balance between neuroepithelial versus neural crest specification. (B) qPCR, (C) western blot, and (D) flow cytometry analysis of <t>SOX1,</t> PAX6, p75, and SOX10 in RBL2 KD and Scramble at day 4 neuroectoderm. (E) Loss of RBL2 in neuroectoderm specification changes the expression of Wnt and Notch pathway components and HoxA-D genes (see Table S1 for full results). (F) Principal-component analysis (see Table S1 for full results). All data are shown as mean ± SD (n = 3). Statistical analysis was performed by two-way ANOVA with multiple comparisons with Tukey correction; ****padjusted < 0.0001, ***padjusted < 0.001, **padjusted < 0.01, *padjusted < 0.05. See also Figures S2 and S3.
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    Figure 2. RBL2 regulates cell fate specification between neuroepithelium and neural crest (A) RBL2 is necessary for neuroectoderm formation. Immunostaining of neuroectoderm markers in Scramble and RBL2 KD cells. Scale bar, 100 mm. (B–D) RBL2 controls the balance between neuroepithelial versus neural crest specification. (B) qPCR, (C) western blot, and (D) flow cytometry analysis of <t>SOX1,</t> PAX6, p75, and SOX10 in RBL2 KD and Scramble at day 4 neuroectoderm. (E) Loss of RBL2 in neuroectoderm specification changes the expression of Wnt and Notch pathway components and HoxA-D genes (see Table S1 for full results). (F) Principal-component analysis (see Table S1 for full results). All data are shown as mean ± SD (n = 3). Statistical analysis was performed by two-way ANOVA with multiple comparisons with Tukey correction; ****padjusted < 0.0001, ***padjusted < 0.001, **padjusted < 0.01, *padjusted < 0.05. See also Figures S2 and S3.
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    Figure 2. RBL2 regulates cell fate specification between neuroepithelium and neural crest (A) RBL2 is necessary for neuroectoderm formation. Immunostaining of neuroectoderm markers in Scramble and RBL2 KD cells. Scale bar, 100 mm. (B–D) RBL2 controls the balance between neuroepithelial versus neural crest specification. (B) qPCR, (C) western blot, and (D) flow cytometry analysis of <t>SOX1,</t> PAX6, p75, and SOX10 in RBL2 KD and Scramble at day 4 neuroectoderm. (E) Loss of RBL2 in neuroectoderm specification changes the expression of Wnt and Notch pathway components and HoxA-D genes (see Table S1 for full results). (F) Principal-component analysis (see Table S1 for full results). All data are shown as mean ± SD (n = 3). Statistical analysis was performed by two-way ANOVA with multiple comparisons with Tukey correction; ****padjusted < 0.0001, ***padjusted < 0.001, **padjusted < 0.01, *padjusted < 0.05. See also Figures S2 and S3.
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    Figure 2. RBL2 regulates cell fate specification between neuroepithelium and neural crest (A) RBL2 is necessary for neuroectoderm formation. Immunostaining of neuroectoderm markers in Scramble and RBL2 KD cells. Scale bar, 100 mm. (B–D) RBL2 controls the balance between neuroepithelial versus neural crest specification. (B) qPCR, (C) western blot, and (D) flow cytometry analysis of <t>SOX1,</t> PAX6, p75, and SOX10 in RBL2 KD and Scramble at day 4 neuroectoderm. (E) Loss of RBL2 in neuroectoderm specification changes the expression of Wnt and Notch pathway components and HoxA-D genes (see Table S1 for full results). (F) Principal-component analysis (see Table S1 for full results). All data are shown as mean ± SD (n = 3). Statistical analysis was performed by two-way ANOVA with multiple comparisons with Tukey correction; ****padjusted < 0.0001, ***padjusted < 0.001, **padjusted < 0.01, *padjusted < 0.05. See also Figures S2 and S3.
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    Figure 2. RBL2 regulates cell fate specification between neuroepithelium and neural crest (A) RBL2 is necessary for neuroectoderm formation. Immunostaining of neuroectoderm markers in Scramble and RBL2 KD cells. Scale bar, 100 mm. (B–D) RBL2 controls the balance between neuroepithelial versus neural crest specification. (B) qPCR, (C) western blot, and (D) flow cytometry analysis of <t>SOX1,</t> PAX6, p75, and SOX10 in RBL2 KD and Scramble at day 4 neuroectoderm. (E) Loss of RBL2 in neuroectoderm specification changes the expression of Wnt and Notch pathway components and HoxA-D genes (see Table S1 for full results). (F) Principal-component analysis (see Table S1 for full results). All data are shown as mean ± SD (n = 3). Statistical analysis was performed by two-way ANOVA with multiple comparisons with Tukey correction; ****padjusted < 0.0001, ***padjusted < 0.001, **padjusted < 0.01, *padjusted < 0.05. See also Figures S2 and S3.
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    Figure 2. RBL2 regulates cell fate specification between neuroepithelium and neural crest (A) RBL2 is necessary for neuroectoderm formation. Immunostaining of neuroectoderm markers in Scramble and RBL2 KD cells. Scale bar, 100 mm. (B–D) RBL2 controls the balance between neuroepithelial versus neural crest specification. (B) qPCR, (C) western blot, and (D) flow cytometry analysis of <t>SOX1,</t> PAX6, p75, and SOX10 in RBL2 KD and Scramble at day 4 neuroectoderm. (E) Loss of RBL2 in neuroectoderm specification changes the expression of Wnt and Notch pathway components and HoxA-D genes (see Table S1 for full results). (F) Principal-component analysis (see Table S1 for full results). All data are shown as mean ± SD (n = 3). Statistical analysis was performed by two-way ANOVA with multiple comparisons with Tukey correction; ****padjusted < 0.0001, ***padjusted < 0.001, **padjusted < 0.01, *padjusted < 0.05. See also Figures S2 and S3.
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    Figure 2. RBL2 regulates cell fate specification between neuroepithelium and neural crest (A) RBL2 is necessary for neuroectoderm formation. Immunostaining of neuroectoderm markers in Scramble and RBL2 KD cells. Scale bar, 100 mm. (B–D) RBL2 controls the balance between neuroepithelial versus neural crest specification. (B) qPCR, (C) western blot, and (D) flow cytometry analysis of SOX1, PAX6, p75, and SOX10 in RBL2 KD and Scramble at day 4 neuroectoderm. (E) Loss of RBL2 in neuroectoderm specification changes the expression of Wnt and Notch pathway components and HoxA-D genes (see Table S1 for full results). (F) Principal-component analysis (see Table S1 for full results). All data are shown as mean ± SD (n = 3). Statistical analysis was performed by two-way ANOVA with multiple comparisons with Tukey correction; ****padjusted < 0.0001, ***padjusted < 0.001, **padjusted < 0.01, *padjusted < 0.05. See also Figures S2 and S3.

    Journal: Cell reports

    Article Title: RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling.

    doi: 10.1016/j.celrep.2023.113146

    Figure Lengend Snippet: Figure 2. RBL2 regulates cell fate specification between neuroepithelium and neural crest (A) RBL2 is necessary for neuroectoderm formation. Immunostaining of neuroectoderm markers in Scramble and RBL2 KD cells. Scale bar, 100 mm. (B–D) RBL2 controls the balance between neuroepithelial versus neural crest specification. (B) qPCR, (C) western blot, and (D) flow cytometry analysis of SOX1, PAX6, p75, and SOX10 in RBL2 KD and Scramble at day 4 neuroectoderm. (E) Loss of RBL2 in neuroectoderm specification changes the expression of Wnt and Notch pathway components and HoxA-D genes (see Table S1 for full results). (F) Principal-component analysis (see Table S1 for full results). All data are shown as mean ± SD (n = 3). Statistical analysis was performed by two-way ANOVA with multiple comparisons with Tukey correction; ****padjusted < 0.0001, ***padjusted < 0.001, **padjusted < 0.01, *padjusted < 0.05. See also Figures S2 and S3.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-human Nanog R&D Systems Cat# AF1997 Mouse anti-human Oct4 Santa Cruz Biotechnology Cat# sc-5279 Goat anti-human SOX2 R&D Systems Cat# AF2018 Rabbit anti-human Eomes Abcam Cat# ab23345 Goat anti-human Brachyury R&D Systems Cat# AF2085 Goat anti-human SOX17 R&D Systems Cat# AF1924 PAX6 rabbit polyclonal Cambridge BioScience Cat# PRB-278P-100 pRb mouse monoclonal BD Pharmingen Cat# 554136 (G3-245) RBL1/p107 (C-18) rabbit polyclonal Santa Cruz Biotechnology Cat# sc-318 RBL2/p130 (C-20) rabbit polyclonal Santa Cruz Biotechnology Cat# sc-317 SOX1 goat polyclonal R&D Systems Cat# AF3369 Actin mouse monoclonal Chemicon Cat# MAB1501 E2F1 (C-20) Santa Cruz Biotechnology Cat# sc-193 E2F4 (A-20) Santa Cruz Biotechnology Cat# sc-1082x SOX1 goat polyclonal R&D Systems Cat# AF3369 p75 (C-20) goat polyclonal Santa Cruz Biotechnology Cat# sc-6188 WNT4 (m-70) rabbit polyclonal Santa Cruz Biotechnology Cat# sc-13962 WNT5A (H-58) rabbit polyclonal Santa Cruz Biotechnology Cat# sc 30224 WNT8A rabbit polyclonal Sigma Cat# SAB1411397 WNT7B goat polyclonal R&D Systems Cat# AF3460 HES5 rabbit polyclonal Abcam Cat# ab25374 DLL1 H-265 rabbit polyclonal Santa Cruz Biotechnology Cat# sc-9102 DLL3 H-110 rabbit polyclonal Santa Cruz Biotechnology Cat# sc-67270 P-ser33-B-cat rabbit polyclonal Santa Cruz Biotechnology Cat# sc-16743-R B-catenin (H-102) rabbit polyclonal Santa Cruz Biotechnology Cat# sc-7199 Beta Tubulin 3/Tuj1 [GT1338] mouse monoclonal Stratech Cat# GTX631831-GTX Histone H3 Abcam Cat# ab1791 Histone H3 (tri methyl K4) Abcam Cat# ab8580 Histone H3 (tri methyl K27) Diagenode Cat# C15200181 (MAb-181-050) Histone H3 (mono methyl K4) Abcam Cat# ab8895 Histone H3 (acetyl K27) Active Motif Cat# 39135 Histone H3 (tri methyl K36) Abcam Cat# ab9050 SMAD2/3 Bio-techne Cat# AF3797 Oct-3/4 (C-10) Santa Cruz Cat# sc-5279 Actin, clone C4 Chemicon Cat# MAB1501 Goat a-mouse IgM Alexa Fluor 647 Invitrogen Cat# A21238 Donkey a-mouse IgG Alexa Fluor 647 Invitrogen Cat# A31571 Donkey a-goat Alexa Fluor 647 Invitrogen Cat# A21447 Mouse Anti-Human CD133-BV786, clone W6B3C1 BD Biosciences Cat# BD 747640 Mouse anti-SSEA-4 Alexa Fluor 647, clone MC813-70 BD Biosciences Cat# BD 560796 Mouse IgG1-BV786, k Isotype Control BD Biosciences Cat# BD 563330 (Continued on next page) 18 Cell Reports 42, 113146, September 26, 2023

    Techniques: Immunostaining, Western Blot, Cytometry, Expressing